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GeneArt? CRISPR Nuclease Vector with CD4 Enrichment Kit
貨號: A21175  規格  Promoter: CMV, U6 Reporter: CD4 Key Function: Gene Editing, Stable Cell Line Development, Genome Engineering, Episomal Expression Product Size: 10 reactions Cloning Method: Restriction Enzyme ? MCS Delivery Method: Transfection Protein Tag or Fusion: CD4 fusion  內容及儲存  Contains:? CRISPR CD4 Nuclease Vector, linearized? 10X Oligonucleotide Annealing Buffer? DNase/RNase-free Water? 5X Ligation Buffer? T4 DNA Ligase? U6 Forward Sequencing Primer? Control Double-stranded OligonucleotideStore in freezer (-5 to -30°C).  描述  The GeneArt® CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads® CD4 magnetic beads. Transfection ...
2015 - 06 - 01
TRIzol? Reagent
貨號: 15596018  規格  Sample Type (General): Bacteria, Yeast, Blood, Plant Samples, Tissue, Cells, Viral Samples High Throughput Compatibility: Not High Throughput-Compatible (Manual) Downstream Application: Reverse Transcriptase PCR (RT-PCR), Cloning, Real-Time Quantitative PCR (qPCR), Nuclease Protection Assays, cDNA Library Construction, Northern Blotting Scale: Scaleable System: TRIzol Quantity: 1 Product Size: 200 mL Final Product: Total RNA, micro RNA, Transcriptome RNA Purification Time: 1 hr Number of Reactions: 200 Preps Isolation Technology: Organic Extraction Starting Material (Amount): Up to 1 x 10^7 cells, Up to 1 g tissue Shipping Condition: Room Temperature  內容及儲存  包括TRIzol® 試劑。置于2°C至8°C保存。  描述  TRIzol® 試劑是一種完全的即用型試劑,可用于提取高質量的總RNA或者從各種生物樣本中同時提取RNA、DNA和蛋白質。這種酚和異硫氰酸胍單相溶液,可用于提取人、動物、植物、酵母或細菌來源的細胞和組織樣本中的純RNA、DNA和蛋白質,只需一個小時即可完成。? 從一份樣品同時提取RNA、DNA和蛋白質? 即便是難以處...
2015 - 05 - 27
GeneArt? CRISPR Nuclease Vector with CD4 Enrichment Kit
貨號: A21175  規格  Promoter: CMV, U6 Reporter: CD4 Key Function: Gene Editing, Stable Cell Line Development, Genome Engineering, Episomal Expression Product Size: 10 reactions Cloning Method: Restriction Enzyme ? MCS Delivery Method: Transfection Protein Tag or Fusion: CD4 fusion  內容及儲存  Contains:? CRISPR CD4 Nuclease Vector, linearized? 10X Oligonucleotide Annealing Buffer? DNase/RNase-free Water? 5X Ligation Buffer? T4 DNA Ligase? U6 Forward Sequencing Primer? Control Double-stranded OligonucleotideStore in freezer (-5 to -30°C).  描述  The GeneArt® CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads® CD4 magnetic beads. Transfection ...
2015 - 06 - 01
Lipofectamine? 2000 Transfection Reagent
貨號: 11668019  規格  Cell Type: Established Cell Lines, Hard-to-Transfect Cells, Stem Cells, Primary Cells Sample Type (Specific): Plasmid DNA, RNAi Plasmids (shRNA, miR), Synthetic siRNA Transfection Technique: Lipid-Based Transfection Serum Compatible: Yes Product Size: 1.5 mL Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice High Throughput Compatibility: High Throughput-Compatible Green Features: Sustainable packaging  內容及儲存  置于+4°C保存。Lipofectamine? 2000試劑正確儲存時可穩定保存6個月。  描述  Lipofectamine® 2000轉染試劑是一種多用途轉染試劑,可在各種貼壁和懸浮細胞系中提供高效轉染。研究人員可使用Lipofectamine® 2000試劑進行基于siRNA和shRNA的基因敲除實驗及基因表達研究。Lipofectamine® 2000是Life Technologies提供的用于siRNA和質粒DNA共轉染的最佳試劑。采用Lipofectamine® 2000試劑,您可以實現:? 在各種細胞系中具有卓越的轉染效率和高重組蛋白表達水平 (表1)? 出眾的siRNA和質粒DNA共轉染性能? 在血清存在時亦具有可靠的轉染效率 – 轉染后無需更換培養基? 適用于高通量應用的可靠性能? 建立穩定細胞系的最佳選擇適用于基因表達和基因沉默的高性能轉染試劑Lipofectamine® 2000試劑適合所有常見細胞系及許多難以轉染的細胞系,可用于含有或不含血清的培養基。在基因沉默應用中...
2015 - 05 - 27
PURELINK RNA MINI KIT
貨號: 12183020 規格 Sample Type (General):Bacteria, Yeast, Blood, Tissue, Plant Samples, CellsHigh Throughput Compatibility:Not High Throughput-Compatible (Manual)Downstream Application:Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction, Nuclease Protection Assays, Next-Generation Sequencing, Microarray Analysis, Real-Time Quantitative PCR (qPCR)Scale:MiniSystem:PureLink?Quantity:10 columnsProduct Size:10 prepsFinal Product:Total RNAYield (up to):1000 µgNumber of Reactions:10 PrepsElution Volume - max:300 µlElution Volume - min:30 µlIsolation Technology:Silica Spin ColumnStarting Material (Amount):Up to 200 mg tissue, 5 x 10^6 to 1 x 10^8 cellsShipping Condition:Room Temperature內容及儲存 The PureLink® RNA Mini Kit comes with enough RNA lysis solution, spin cartridges, wash tubes, recovery tubes, wash buffers, and RNase-free water to perform 10 RNA purifications. All components sho...
2015 - 05 - 25
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貨號: 10378016   規格  Validated Application: Prevention of Cell Culture Contamination Agent: L-Glutamine, Streptomycin, Penicillin Form: Liquid Reagent Type: Antibiotic, Supplement Concentrated: 100 X Product Size: 100 mL Concentration: 50 mg?mL Shipping Condition: Dry Ice  描述  在 10 mM 檸檬酸鹽緩沖液中含有 10000 單位的青霉素(堿)、10000 µg 的鏈霉素(堿)以及 29.2 mg L-谷氨酰胺/ml(0.85% 鹽水),以保持青霉素效力。 使用青霉素 G(鈉鹽)和鏈霉素硫酸鹽。抗菌譜: 革蘭氏陽性和革蘭氏陰性菌。
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